Central Proteomics Facility (CPF)
The CPF consists of two proteomics facilities, one located in the Old Road Campus (Headington) and the second site at the Dunn School of Pathology (South Parks Road) . The major goal of the Headington-based site is to provide a proteomics platform for the research community located at the Headington campus. The WTCHG (Churchill campus) and WIMM (John Radcliffe campus) are providing lab space for the proteomics equipment. In addition, a proteomics analysis pipeline and a MASCOT proteomics server have been created in collaboration with the Computational Biology Research Group (CBRG). For specific inquiries, please contact us.
Clinical Proteomics
The CPF (Headington Site) has established a number of collaborations with clinical researchers on campus, which includes the development of proteomics based approaches for the discovery of potential prognostic and diagnostic biomarkers.
Proteomics and mass spectrometry
The current proteomics platform includes equipment for 1D and 2D gel electrophoresis and protein purification (HPLC and FPLC). The following mass spectrometers are operational: an Orbitrap Velos (ThermoTM) coupled to a nano Acquity nUPLC (WatersTM), a nanoflow LC-MS/MS system (HCTplusTM, Bruker Daltonics) coupled to a HPLC system (Ultimate3000TM, Dionex/LC-Packings), and a MALDI-TOF/TOF instrument (UltraflexTM, Bruker Daltonics). These technology platforms are complemented by a nano UPLC-MS/MS (QTofpremierTM, Waters) and nano-LC-CHIP-MS/MS (QTof 6520TM, Agilent) systems for quantitative and comparative proteomics applications as well as clinical biomarkers discovery (Protein expression profiling system). Also, we have available a nano-LC CHIP QQQ-MS (6460TM, Agilent) triple quadrupole mass spectrometer for quantitative meausurements of defined biomolecules using multiple reaction monitoring (MRM).
The equipment is operated by dedicated specialists. We are integrating these technologies into our own research, but have also established collaborations with other investigators on campus. We are particularly interested in exploring new ways to characterize isolated protein complexes at a very low level of abundance. For this purpose, we use either 2D gel electrophoresis or 2D/3D liquid chromatography (LC) workflows including OFFGELTM (Agilent) based separation prior to analysis by MS, in order to identify protein components and post-translational modifications with very low stoechiometries.
Please contact us for any requests for proteomics based applications and sample analysis.